1TAS

CRYSTALLINE MITOCHONDRIAL ASPARTATE AMINOTRANSFERASE EXISTS IN ONLY TWO CONFORMATIONS

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	57.400, 52.400, 136.900
Unit cell angles	90.00, 101.50, 90.00

Refinement procedure

Resolution	15.000 - 2.800
R-factor	0.16
RMSD bond length	0.020
RMSD bond angle	2.800
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.800 *	2.800 *
Rmerge	0.074 *
Total number of observations	44737 *
Number of reflections	17636 *
Completeness [%]	87.9 *	83.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	D,L-2-methyl aspartate	80 (mM)
3	1	drop	sodium phosphate	20 (mM)	pH7.5
4	1	drop	PEG	8-14 (%(w/v))
5	1	reservoir	PEG	16-28 (%(w/v))