1TAL

ALPHA-LYTIC PROTEASE AT 120 K (SINGLE STRUCTURE MODEL)

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1992-07-25
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	65.800, 65.800, 79.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 1.500
R-factor	0.165
Rwork	0.165
R-free	0.19200
Structure solution method	REFINEMENT OF 2ALP
RMSD bond length	0.009
RMSD bond angle	1.310 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.570
High resolution limit [Å]	1.500	1.500
Rmerge	0.059
Total number of observations	148867 *
Number of reflections	31458 *
Completeness [%]	99.0	99.1
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		pH 8.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		1.3 (M)
3	1	reservoir	Tris-HCl	20 (mM)