1TAG
STRUCTURAL DETERMINANTS FOR ACTIVATION OF THE ALPHA-SUBUNIT OF A HETEROTRIMERIC G PROTEIN
Experimental procedure
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 71.900, 87.800, 143.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.21100 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.460 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 * |
| Rmerge | 0.124 * |
| Number of reflections | 41071 |
| Completeness [%] | 98.3 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13.3 (mg/ml) | |
| 10 | 1 | reservoir | 200 (mM) | ||
| 11 | 1 | reservoir | beta-mercaptoethanol | 0.1 (%) | |
| 2 | 1 | drop | PEG8000 | 6 (%) | |
| 3 | 1 | drop | 6.67 (%) | ||
| 4 | 1 | drop | MES | 33.3 (mM) | |
| 5 | 1 | drop | 133 (mM) | ||
| 6 | 1 | drop | beta-mercaptoethanol | 0.1 (%) | |
| 7 | 1 | reservoir | PEG8000 | 9 (%) | |
| 8 | 1 | reservoir | glycerol | 10 (%) | |
| 9 | 1 | reservoir | MES | 50 (mM) |
