1TA1

H141C mutant of rat liver arginase I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	110
Detector technology	CCD
Collection date	2000-05-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.946
Spacegroup name	P 32
Unit cell lengths	87.357, 87.357, 105.656
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.500 - 2.500
R-factor	0.214
Rwork	0.214
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	30722
Completeness [%]		99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	bicine, PEG8000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K