1T9X
Structural Basis of Multidrug Transport by the AcrB Multidrug Efflux Pump
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | H 3 2 |
Unit cell lengths | 144.967, 144.967, 519.372 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 3.280 - 3.080 |
R-factor | 0.27392 |
Rwork | 0.272 |
R-free | 0.35893 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 182.570 | 3.280 |
High resolution limit [Å] | 3.080 | 3.080 |
Number of reflections | 65233 | |
Completeness [%] | 100.0 | 97.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |