1T9W

Structural Basis of Multidrug Transport by the AcrB Multidrug Efflux Pump

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	H 3 2
Unit cell lengths	144.508, 144.508, 519.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	182.570 - 3.230
R-factor	0.27043
Rwork	0.268
R-free	0.34865
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	182.570	3.380
High resolution limit [Å]	3.230	3.230
Number of reflections	42277
Completeness [%]	96.8	94.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1