1T9T
Structural Basis of Multidrug transport by the AcrB Multidrug Efflux Pump
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | H 3 2 |
Unit cell lengths | 144.365, 144.365, 518.929 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 182.570 - 3.230 |
R-factor | 0.27677 |
Rwork | 0.275 |
R-free | 0.33841 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 183.000 | 3.380 |
High resolution limit [Å] | 3.230 | 3.230 |
Number of reflections | 42155 | |
Completeness [%] | 99.6 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |