1T9F

Structural genomics of Caenorhabditis elegans: Structure of a protein with unknown function

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-21
Detector	MARRESEARCH
Wavelength(s)	1.47
Spacegroup name	P 64
Unit cell lengths	76.376, 76.376, 57.388
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.790 - 2.000
R-factor	0.217
Rwork	0.217
R-free	0.26700
Structure solution method	SAD
Starting model (for MR)	from SAD map based on Gd-derivative
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.049	0.168
Number of reflections	12971
<I/σ(I)>	13.4	5.4
Completeness [%]	99.9	100
Redundancy	5.7	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	2.1M ammonium sulfate, 0.1M HEPES, 6% PEG400, 1% dioxane, 5 mM DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	7	295	2.1M ammonium sulfate, 0.1M HEPES, 6% PEG400, 1% dioxane, 5 mM DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K