1T95
Crystal Structure of the Shwachman-Bodian-Diamond Syndrome Protein Orthologue from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97920, 0.979289, 0.891971 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.668, 44.448, 54.720 |
| Unit cell angles | 75.87, 85.61, 69.49 |
Refinement procedure
| Resolution | 52.700 - 1.900 |
| R-factor | 0.21358 |
| Rwork | 0.212 |
| R-free | 0.24920 |
| Structure solution method | MAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.761 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 52.710 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 22129 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 297 | PEG 8000, CHES, DTT, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
