1T91
crystal structure of human small GTPase Rab7(GTP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9722 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.695, 56.777, 74.510 |
| Unit cell angles | 71.51, 71.72, 77.75 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.2493 |
| Rwork | 0.243 |
| R-free | 0.24730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | mouse rab5c 1huq |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.712 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.049 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.047 | |
| Number of reflections | 61851 | |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 94.5 | 92.9 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | polyethylene glycol monomethyl ether 2000, ammonium sulfate, MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
