1T8Y
Crystal Structure of E.coli AMP Nucleosidase complexed with phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 4 |
| Unit cell lengths | 268.500, 268.500, 114.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.070 - 3.000 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.190 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 78160 | |
| Completeness [%] | 96.0 | 89.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 291 | ammonium formate, sodium chloride, ammonium phosphate, ethylene glycol, sodium citrate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
