1T8W

Crystal Structure of E. coli AMP Nucleosidase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 8-BM
Synchrotron site	APS
Beamline	8-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2003-04-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	I 4
Unit cell lengths	271.800, 271.800, 113.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.930 - 2.800
R-factor	0.223
Rwork	0.223
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.980
High resolution limit [Å]	2.800	2.800
Number of reflections	95360
<I/σ(I)>	28.1
Completeness [%]	93.7	85.9
Redundancy	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	ammonium formate, sodium chloride, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	7	291	ammonium formate, sodium chloride, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K