1T8T
Crystal Structure of human 3-O-Sulfotransferase-3 with bound PAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.338, 155.394, 93.323 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.850 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1S6T |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 49160 | |
| <I/σ(I)> | 23.1 | 3.5 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 3.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | micro-seeding | 5.5 | 297 | sodium citrate, ammonium acetate, PEG 4000, PAP, pH 5.5, micro-seeding, temperature 297K |
