1T8R

Crystal Structure of E. coli AMP Nucleosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 8-BM
Synchrotron site	APS
Beamline	8-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2003-04-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 42 21 2
Unit cell lengths	246.630, 246.630, 111.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.220 - 2.700
R-factor	0.219
Rwork	0.219
R-free	0.24600
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.840
High resolution limit [Å]	2.700	2.700
Number of reflections	90572
<I/σ(I)>	26.8
Completeness [%]	99.3	100
Redundancy	17.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	291	ammonium formate, sodium chloride, HEPES, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K