1T8K

Crystal Structure of apo acyl carrier protein from E. coli

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.98,1.28242,1.28295,1.21940
Spacegroup name	P 21 21 21
Unit cell lengths	27.510, 41.260, 62.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.100
R-factor	0.13381
Rwork	0.133
R-free	0.14987
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.501
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.140
High resolution limit [Å]	1.100	1.100
Rmerge	0.067	0.199
Number of reflections	46801
<I/σ(I)>	17.1	2.6
Completeness [%]	83.9	36.7
Redundancy	3.7	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	300	peg, zinc acetate, imidazole buffer, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 300K