1T8I
Human DNA Topoisomerase I (70 Kda) In Complex With The Poison Camptothecin and Covalent Complex With A 22 Base Pair DNA Duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.363, 114.423, 74.118 |
| Unit cell angles | 90.00, 93.49, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.24356 |
| Rwork | 0.241 |
| R-free | 0.29154 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4t |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.859 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 16492 | |
| <I/σ(I)> | 13.7 | 1.7 |
| Completeness [%] | 91.0 | 47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 289 | PEG 8000, ammonium sulfate, MES, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | ammonium sulfate | ||
| 3 | 1 | 1 | MES | ||
| 4 | 1 | 2 | ammonium sulfate | ||
| 5 | 1 | 2 | MES |
