1T8H
1.8 A CRYSTAL STRUCTURE OF AN UNCHARACTERIZED B. STEAROTHERMOPHILUS PROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97985 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.970, 48.879, 135.041 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.800 |
| Rwork | 0.180 |
| R-free | 0.21000 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.550 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.880 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.076 | 0.096 |
| Number of reflections | 23429 | |
| <I/σ(I)> | 16.62 | 9.2 |
| Completeness [%] | 81.2 | 64.4 |
| Redundancy | 4.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1M HEPES, 0.5M NaCl, 5mM beta-mercaptoethanol, 25% PEG 6K, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
