1T89

CRYSTAL STRUCTURE OF A HUMAN TYPE III FC GAMMA RECEPTOR IN COMPLEX WITH AN FC FRAGMENT OF IGG1 (HEXAGONAL)

Replaces:  1IIX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	P 65 2 2
Unit cell lengths	114.984, 114.984, 301.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 3.500
Rwork	0.247
R-free	0.30900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1FNL AND 1FC1
RMSD bond length	0.009
RMSD bond angle	1.580
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	3.600
High resolution limit [Å]	3.500	3.500
Number of reflections	15541
<I/σ(I)>	20.2	4
Completeness [%]	98.7	99.4
Redundancy	4.7	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	298	PEG4000, HEPES, pH 6.0, VAPOR DIFFUSION, temperature 298.0K