1T86
Crystal Structure of the Ferrous Cytochrome P450cam Mutant (L358P/C334A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.010, 101.170, 72.920 |
| Unit cell angles | 90.00, 108.30, 90.00 |
Refinement procedure
| Resolution | 32.650 - 1.900 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond angle | 21.200 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.650 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.076 | 0.391 |
| Number of reflections | 61668 | |
| <I/σ(I)> | 19.5 | 2.38 |
| Completeness [%] | 99.7 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 290 | PEG 4000, potassium chloride, Tris-HCl, D-camphor, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
