1T80

Zn-alpha-2-glycoprotein; CHO-ZAG PEG 200

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1271
Spacegroup name	P 43 21 2
Unit cell lengths	122.545, 122.545, 65.445
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
Rwork	0.236
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t7v
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.069	0.524
Number of reflections	29553
<I/σ(I)>	16.5	2.7
Completeness [%]	99.7	99.9
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	298	Ammonium sulfate, PEG 200, HEPES, pH 7.5, Microbatch, temperature 298.0K