1T7W

Zn-alpha-2-glycoprotein; CHO-ZAG PEG 400

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-19
Detector	ADSC QUANTUM 210
Wavelength(s)	1.100
Spacegroup name	P 43 21 2
Unit cell lengths	121.681, 121.681, 65.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.700
Rwork	0.232
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t7v
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.100	0.456
Number of reflections	13782
<I/σ(I)>	12.1	1.8
Completeness [%]	98.5	95.6
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	298	Ammonium sulfate, PEG 400, HEPES, pH 7.5, Microbatch, temperature 298.0K