1T7S
Structural Genomics of Caenorhabditis elegans: Structure of BAG-1 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979244 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 75.518, 86.164, 125.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.630 - 2.800 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.29800 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 19481 | |
| <I/σ(I)> | 12.3 | 3.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 295 | 1.6M ammonium sulfate, 0.1M MES, 5% dioxane, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
