1T72
Crystal structure of phosphate transport system protein phoU from Aquifex aeolicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 43 |
| Unit cell lengths | 113.500, 113.500, 155.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.900 |
| R-factor | 0.21637 |
| Rwork | 0.201 |
| R-free | 0.29271 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.381 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | |
| High resolution limit [Å] | 2.795 | 2.795 |
| Rmerge | 0.092 | 0.127 |
| Number of reflections | 47223 | |
| <I/σ(I)> | 16 | 21.6 |
| Completeness [%] | 98.0 | 84.55 |
| Redundancy | 3.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | sodium chloride, lithium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
