1T6V
Crystal structure analysis of the nurse shark new antigen receptor (NAR) variable domain in complex with lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1159 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.790, 113.810, 58.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.100 - 1.700 |
| R-factor | 0.19527 |
| Rwork | 0.194 |
| R-free | 0.22076 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sq2 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.867 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.100 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 54344 | |
| <I/σ(I)> | 14.1 | 2.3 |
| Completeness [%] | 96.5 | 97.7 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 15% PEG 10,000, 0.1M imidazole malate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
