1T6K
Crystal structure of phzF from Pseudomonas fluorescens 2-79
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-02-24 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 56.220, 56.220, 155.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.18774 |
| Rwork | 0.186 |
| R-free | 0.22209 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1P9V |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.833 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK |
| Phasing software | RESOLVE |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.047 | 0.175 |
| Number of reflections | 27247 | |
| <I/σ(I)> | 19.4 | 4.7 |
| Completeness [%] | 99.7 | 96.9 |
| Redundancy | 4.9 | 2.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | Reservoir solution: 0.20 M ammonium sulfate, 0.10 M sodium citrate pH 5.6 and 18% (w/v) PEG 4000. The protein concentration was 17 mg/ml, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
