1T6I
Nickel Superoxide Dismutase (NiSOD) Apo Structure
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.911656, 0.97912, 0.979311 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 60.430, 110.241, 110.659 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.810 |
| R-factor | 0.238 |
| Rwork | 0.235 |
| R-free | 0.29200 |
| Structure solution method | MAD |
| RMSD bond length | 0.130 |
| RMSD bond angle | 1.380 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 9300 | |
| <I/σ(I)> | 22.4 | 4.3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | MPEG 2000, Hepes, methanol, calcium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
