1T68
Crystal structure of nitrophorin 2 complex with NO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 39.295, 125.444, 33.196 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.800 - 1.450 |
| R-factor | 0.19488 |
| Rwork | 0.193 |
| R-free | 0.23462 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1euo |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.989 |
| Data reduction software | XDS |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.800 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.093 | 0.246 |
| Number of reflections | 26809 | |
| <I/σ(I)> | 13.2 | 2.3 |
| Completeness [%] | 89.3 | 98.1 |
| Redundancy | 4.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | sodium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
