1T66
The structure of FAB with intermediate affinity for fluorescein.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.640, 117.120, 154.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.000 - 2.300 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1flr |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.770 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.100 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.104 | 0.533 |
| Number of reflections | 49354 | |
| <I/σ(I)> | 8.8 | 1.8 |
| Completeness [%] | 97.6 | 95.8 |
| Redundancy | 4.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 286 | ammonium sulphate, sodium phosphate, pH 6.2, batch, temperature 286K, pH 6.20 |
