1T5P
Human Heme Oxygenase Oxidation of alpha- and gamma-meso-phenylhemes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 119 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-02 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.914, 64.425, 60.467 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.110 |
| Rwork | 0.270 |
| R-free | 0.31000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qq8 |
| RMSD bond length | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.065 | 0.039 |
| Number of reflections | 21619 | |
| <I/σ(I)> | 21.2 | 2.12 |
| Completeness [%] | 91.8 | |
| Redundancy | 9.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 301 | NH4SO4, hepes, hexanediol, Jeffamine-600 and water, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 301K |
