1T5J
Crystal structure of ribosylglycohydrolase MJ1187 from Methanococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 69.775, 69.775, 139.724 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.700 |
| Rwork | 0.204 |
| R-free | 0.26500 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.940 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.074 | 0.305 |
| Number of reflections | 9961 | |
| <I/σ(I)> | 13.7 | 4 |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 2.75M NaFormate, 0.1M Tris pH 8.5 , VAPOR DIFFUSION, HANGING DROP, temperature 298K |
