1T56
Crystal structure of TetR family repressor M. tuberculosis EthR
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | .9393 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 121.500, 121.500, 33.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.20836 |
| Rwork | 0.208 |
| R-free | 0.22150 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.924 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.085 | 0.296 |
| Number of reflections | 27776 | |
| <I/σ(I)> | 5.3 | 2.6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 12.7 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.75 | 287 | ammonium sulphate, 1,4-dioxane, glycerol, 1-(4-morpholino)ethanesulfonic acid, pH 6.75, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
