1T4S
arginase-L-valine complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2001-02-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.982 |
Spacegroup name | P 32 |
Unit cell lengths | 87.800, 87.800, 110.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.740 - 2.800 |
R-factor | 0.258 |
Rwork | 0.258 |
R-free | 0.29200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 26074 | |
Completeness [%] | 99.6 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | bicine, PEG8000, L-valine, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |