1T4K

Crystal Structure of Unliganded Aldolase Antibody 93F3 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-01
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	79.701, 81.228, 149.496
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.233
Rwork	0.233
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fgw
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	31622
<I/σ(I)>	17.4	3.5
Completeness [%]	91.3	83.7
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5	298	16-20% (w/v) PEG 8000, 0.3 M zinc acetate, 0.1 M sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 5.50