1T4C
Formyl-CoA Transferase in complex with Oxalyl-CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-02-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.087 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 100.163, 100.163, 196.872 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.610 |
| R-factor | 0.20369 |
| Rwork | 0.200 |
| R-free | 0.27308 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p5r |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.821 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.480 | 2.670 |
| High resolution limit [Å] | 2.610 | 2.600 |
| Number of reflections | 30208 | |
| <I/σ(I)> | 15.6 | 4.6 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 6.4 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | Mg acetate, PEG 8000, Na cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
