1T4A
Structure of B. Subtilis PurS C2 Crystal Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2003-08-19 |
| Detector | ADSC QUANTUM 310 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.128, 42.206, 47.033 |
| Unit cell angles | 90.00, 118.22, 90.00 |
Refinement procedure
| Resolution | 19.620 - 2.000 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.287 |
| Number of reflections | 19509 | |
| <I/σ(I)> | 20 | 10 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 20% PEG4K, 5% glycerol, 15% isopropanol, 100mM sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
