1T43
Crystal Structure Analysis of E.coli Protein (N5)-Glutamine Methyltransferase (HemK)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0090,1.0069 |
| Spacegroup name | P 63 |
| Unit cell lengths | 138.933, 138.933, 40.583 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.200 |
| R-factor | 0.289 |
| Rwork | 0.289 |
| R-free | 0.31500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nv8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.300 |
| High resolution limit [Å] | 3.190 | 3.190 |
| Rmerge | 0.075 | 0.250 |
| Number of reflections | 7675 | |
| <I/σ(I)> | 14.8 | 6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 289 | Ammonium sulfate, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
