1T3U

Unknown conserved bacterial protein from Pseudomonas aeruginosa PAO1

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-31
Detector	MARRESEARCH
Wavelength(s)	0.9790
Spacegroup name	P 41
Unit cell lengths	106.726, 106.726, 36.454
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.970 - 2.500
R-factor	0.236
Rwork	0.236
R-free	0.29000
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	28501
<I/σ(I)>	11	1.75
Completeness [%]	91.4	79
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	0.1M SODIUM CITRATE, 17% ISOPROPANOL, 9% PEG 4K, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K