1T3J

Mitofusin domain HR2 V686M/I708M mutant

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-01-22
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9795, 0.9797, 0.9641, 0.9800
Spacegroup name	F 41 3 2
Unit cell lengths	162.830, 162.830, 162.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.860 - 2.500
Rwork	0.241
R-free	0.28600
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.520	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.079	0.463
Number of reflections	6772
<I/σ(I)>	25.2	5.6
Completeness [%]	99.5	99.2
Redundancy	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.7	290	Isopropanol, TRIS, Ammonium Acetate, PEG 200, pH 7.7, VAPOR DIFFUSION, SITTING DROP, temperature 295K, temperature 290K