1T3I
Structure of slr0077/SufS, the Essential Cysteine Desulfurase from Synechocystis PCC 6803
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.386, 89.308, 141.815 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.590 - 1.800 |
| R-factor | 0.194 |
| Rwork | 0.191 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | polyalanine version of 1JF9.pdb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.590 | 1.980 |
| High resolution limit [Å] | 1.790 | 1.800 |
| Number of reflections | 94984 | |
| <I/σ(I)> | 18.1 | 4.5 |
| Completeness [%] | 90.9 | 83.3 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277.15 | 0.1M tris pH 8.0, 0.25M MgCl2, 25% PEG 4000, n-octanoylsucrose, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
