1T3E
Structural basis of dynamic glycine receptor clustering
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 61 |
| Unit cell lengths | 161.547, 161.547, 126.252 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.250 |
| R-factor | 0.2458 |
| Rwork | 0.243 |
| R-free | 0.30306 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Unbound gephyrin (not published) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.237 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.330 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.108 | 0.494 |
| Number of reflections | 27162 | |
| <I/σ(I)> | 7.8 | 1.6 |
| Completeness [%] | 97.7 | 97.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | Li2SO4, pH 6., VAPOR DIFFUSION, HANGING DROP, temperature 293K |
