1T3B
X-ray Structure of DsbC from Haemophilus influenzae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2002-07-19 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 74.920, 74.920, 103.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.249 |
| Rwork | 0.246 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eej |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.407 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.570 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.042 | 0.284 |
| Number of reflections | 7826 | |
| <I/σ(I)> | 20.1 | 3.4 |
| Completeness [%] | 71.3 | 35.7 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 4000, sodium phosphate, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
