1T35
CRYSTAL STRUCTURE OF A HYPOTHETICAL PROTEIN YVDD- A PUTATIVE LYSINE DECARBOXYLASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 117.562, 170.773, 89.721 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.720 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.28600 |
| Structure solution method | SAD |
| Starting model (for MR) | EXPERIMENTAL ELECTRON DENSITY |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 92393 | |
| <I/σ(I)> | 10.82 | 1.787 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 2.2 | 2.21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 4% PEG6K, 0.1M MES, 0.3M AMMONIUM SULFATE, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 5.5 |
