1T34
ROTATION MECHANISM FOR TRANSMEMBRANE SIGNALING BY THE ATRIAL NATRIURETIC PEPTIDE RECEPTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-22 |
| Detector | SBC-2 |
| Wavelength(s) | 0.979338 |
| Spacegroup name | P 61 |
| Unit cell lengths | 100.131, 100.131, 259.803 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.710 - 2.950 |
| R-factor | 0.23745 |
| Rwork | 0.237 |
| R-free | 0.26857 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dp4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.550 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.710 | 3.040 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.054 | 0.554 |
| Number of reflections | 25503 | |
| <I/σ(I)> | 30.9 | 3.2 |
| Completeness [%] | 93.7 | 89.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | AMMONIUM SULFATE, SODIUM CHLORIDE, pH 6.5, VAPOR DIFFUSION, HANGING DROP |
