1T33
Structural Genomics, The crystal structure of a putative transcriptional repressor (TetR/AcrR family) from Salmonella typhimurim LT2
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-02 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795, 0.9798, 0.9465 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 110.630, 122.192, 108.991 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.630 - 2.200 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.25300 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.426 |
| Number of reflections | 73137 | |
| <I/σ(I)> | 16.5 | 2.39 |
| Completeness [%] | 99.2 | 93.8 |
| Redundancy | 4.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | 0.1M sodium acetate, 0.17M ammonium acetate, 10% PEG3350, 15% glycerol, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
