1T2I

T76W mutant of RNase Sa from Streptomyces aureofaciens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-10-01
Detector	MARRESEARCH
Wavelength(s)	1.096
Spacegroup name	P 21 21 21
Unit cell lengths	33.872, 40.462, 56.638
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.000 - 1.100
R-factor	0.1303
Rwork	0.128
R-free	0.16698
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rgg
RMSD bond length	0.010
RMSD bond angle	1.428
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	1.110
High resolution limit [Å]	1.100	1.100
Rmerge	0.030	0.180
Number of reflections	29254
<I/σ(I)>	53.3	8.7
Completeness [%]	95.3	90.2
Redundancy	6.4	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	ammonium sulfate, disodium phosphate, monosodium phosphate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K