1T2I
T76W mutant of RNase Sa from Streptomyces aureofaciens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-10-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.096 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.872, 40.462, 56.638 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.000 - 1.100 |
R-factor | 0.1303 |
Rwork | 0.128 |
R-free | 0.16698 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rgg |
RMSD bond length | 0.010 |
RMSD bond angle | 1.428 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 | 1.110 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.030 | 0.180 |
Number of reflections | 29254 | |
<I/σ(I)> | 53.3 | 8.7 |
Completeness [%] | 95.3 | 90.2 |
Redundancy | 6.4 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | ammonium sulfate, disodium phosphate, monosodium phosphate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |