Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-18 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 0.9791, 0.9794, 0.9500 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.914, 73.773, 48.185 |
| Unit cell angles | 90.00, 114.73, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.200 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.25000 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.045 | 0.081 |
| Number of reflections | 38629 | |
| <I/σ(I)> | 20.2 | |
| Completeness [%] | 90.1 | 67.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | PEG 4000, sodium acetate, Tris-HCL, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
