1T1V
Crystal Structure of the Glutaredoxin-like Protein SH3BGRL3 at 1.6 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.624, 80.380, 34.660 |
| Unit cell angles | 90.00, 93.96, 90.00 |
Refinement procedure
| Resolution | 34.500 - 1.600 |
| R-factor | 0.20199 |
| Rwork | 0.197 |
| R-free | 0.24426 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j0f |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.012 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.500 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.098 | 0.331 |
| Number of reflections | 18915 | |
| <I/σ(I)> | 13.1 | 5.3 |
| Completeness [%] | 99.4 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | Ammonium sulfate, Na-acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
