1T1N
CRYSTAL STRUCTURE OF CARBONMONOXY HEMOGLOBIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-04-15 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.170, 88.060, 55.250 |
| Unit cell angles | 90.00, 97.65, 90.00 |
Refinement procedure
| Resolution | 16.000 - 2.200 |
| R-factor | 0.192 * |
| Rwork | 0.192 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pbx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 21.790 * |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.077 | |
| Total number of observations | 68936 * | |
| Number of reflections | 25207 | |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 8 | 4 * | pH 8.0 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | tris | ||
| 2 | 1 | 1 | (NH4)2SO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | protein | 20 (mg/ml) | ||
| 2 | 1 | Tris-HCl | 50 (mM) | ||
| 3 | 1 | 2 | ammonium sulfate | 2.0 (M) |
