1T1L

Crystal structure of the long-chain fatty acid transporter FadL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 8-BM
Synchrotron site	APS
Beamline	8-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-08
Detector	ADSC QUANTUM 315
Wavelength(s)	1.07, 0.97
Spacegroup name	P 31 2 1
Unit cell lengths	122.020, 122.020, 164.736
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	11.990 - 2.800
R-factor	0.306
Rwork	0.298
R-free	0.33100
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.030	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	36854
Completeness [%]	93.6
Redundancy	3.3	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	295	Ammonium Formate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	6.8	295	Ammonium Formate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K