1T1J
Crystal structure of genomics APC5043
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.96411 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 66.500, 66.500, 116.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.980 - 1.700 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.22900 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.980 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.052 | 0.844 |
| Number of reflections | 59550 | |
| <I/σ(I)> | 7.7 | 1.42 |
| Completeness [%] | 93.8 | 99.9 |
| Redundancy | 6.2 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 300 | 3.5M Sodium formate, 4% Ethylene, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
